Impact of the oxidized guanine lesion spiroiminodihydantoin on the conformation and thermodynamic stability of a 15-mer DNA duplex.
Identifieur interne : 002976 ( Main/Exploration ); précédent : 002975; suivant : 002977Impact of the oxidized guanine lesion spiroiminodihydantoin on the conformation and thermodynamic stability of a 15-mer DNA duplex.
Auteurs : Fadzai Chinyengetere [États-Unis] ; Elizabeth R. JamiesonSource :
- Biochemistry [ 0006-2960 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN (), Adduits à l'ADN (), Calorimétrie différentielle à balayage, Concentration osmolaire, Conformation d'acide nucléique, Dichroïsme circulaire, Dénaturation d'acide nucléique, Guanine (analogues et dérivés), Guanine (métabolisme), Guanosine (), Guanosine (analogues et dérivés), Guanosine (physiologie), Hétéroduplexes d'acides nucléiques (), Modèles biologiques, Mutation (physiologie), Oxydoréduction, Spiranes (), Température, Thermodynamique.
- MESH :
- analogues et dérivés : Guanine, Guanosine.
- métabolisme : Guanine.
- physiologie : Guanosine, Mutation.
- ADN, Adduits à l'ADN, Calorimétrie différentielle à balayage, Concentration osmolaire, Conformation d'acide nucléique, Dichroïsme circulaire, Dénaturation d'acide nucléique, Guanosine, Hétéroduplexes d'acides nucléiques, Modèles biologiques, Oxydoréduction, Spiranes, Température, Thermodynamique.
English descriptors
- KwdEn :
- Calorimetry, Differential Scanning, Circular Dichroism, DNA (chemistry), DNA Adducts (chemistry), Guanine (analogs & derivatives), Guanine (metabolism), Guanosine (analogs & derivatives), Guanosine (chemistry), Guanosine (physiology), Models, Biological, Mutation (physiology), Nucleic Acid Conformation, Nucleic Acid Denaturation, Nucleic Acid Heteroduplexes (chemistry), Osmolar Concentration, Oxidation-Reduction, Spiro Compounds (chemistry), Temperature, Thermodynamics.
- MESH :
- chemical , analogs & derivatives : Guanine, Guanosine.
- chemical , chemistry : DNA, DNA Adducts, Guanosine, Nucleic Acid Heteroduplexes, Spiro Compounds.
- chemical , metabolism : Guanine.
- chemical , physiology : Guanosine.
- physiology : Mutation.
- Calorimetry, Differential Scanning, Circular Dichroism, Models, Biological, Nucleic Acid Conformation, Nucleic Acid Denaturation, Osmolar Concentration, Oxidation-Reduction, Temperature, Thermodynamics.
Abstract
Spiroiminodihydantoin (Sp) is a hyperoxidized guanine base produced from oxidation of the mutagenic DNA lesion 7,8-dihydro-8-oxo-2'-deoxguanosine (8-oxoG) by a variety of species including peroxynitrite, singlet oxygen, and the high-valent metals Ir(IV) and Cr(V). In this study, the conformation and thermodynamic stability of a 15-mer DNA duplex containing an Sp lesion are examined using spectroscopic techniques and differential scanning calorimetry (DSC). The Sp lesion does not alter the global B-form conformation of the DNA duplex as determined by circular dichroism spectroscopy. Thermal denaturation experiments find that Sp significantly lowers the thermal stability of the duplex by approximately 20 degrees C. The enthalpies, entropies, and free energies of duplex formation for 15-mers containing guanine, 8-oxoG, and Sp were determined by performing DSC experiments as well as van't Hoff analysis of UV melting spectroscopic data. The thermodynamic stability of the Sp duplex is significantly reduced compared to that of both the 8-oxoG and parent G duplexes, with the thermodynamic destabilization being enthalpic in origin. The thermodynamic impact of the Sp lesion is compared to what is found for other types of DNA base damage and discussed in relation to how the presence of this lesion could affect cellular processes, in particular the recognition and repair of these adducts by the base excision repair enzymes.
DOI: 10.1021/bi701502t
PubMed: 18281959
Affiliations:
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Le document en format XML
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<term>DNA (chemistry)</term>
<term>DNA Adducts (chemistry)</term>
<term>Guanine (analogs & derivatives)</term>
<term>Guanine (metabolism)</term>
<term>Guanosine (analogs & derivatives)</term>
<term>Guanosine (chemistry)</term>
<term>Guanosine (physiology)</term>
<term>Models, Biological</term>
<term>Mutation (physiology)</term>
<term>Nucleic Acid Conformation</term>
<term>Nucleic Acid Denaturation</term>
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<term>Osmolar Concentration</term>
<term>Oxidation-Reduction</term>
<term>Spiro Compounds (chemistry)</term>
<term>Temperature</term>
<term>Thermodynamics</term>
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<term>Adduits à l'ADN ()</term>
<term>Calorimétrie différentielle à balayage</term>
<term>Concentration osmolaire</term>
<term>Conformation d'acide nucléique</term>
<term>Dichroïsme circulaire</term>
<term>Dénaturation d'acide nucléique</term>
<term>Guanine (analogues et dérivés)</term>
<term>Guanine (métabolisme)</term>
<term>Guanosine ()</term>
<term>Guanosine (analogues et dérivés)</term>
<term>Guanosine (physiologie)</term>
<term>Hétéroduplexes d'acides nucléiques ()</term>
<term>Modèles biologiques</term>
<term>Mutation (physiologie)</term>
<term>Oxydoréduction</term>
<term>Spiranes ()</term>
<term>Température</term>
<term>Thermodynamique</term>
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<term>Guanosine</term>
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<term>Spiro Compounds</term>
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<term>Nucleic Acid Denaturation</term>
<term>Osmolar Concentration</term>
<term>Oxidation-Reduction</term>
<term>Temperature</term>
<term>Thermodynamics</term>
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<front><div type="abstract" xml:lang="en">Spiroiminodihydantoin (Sp) is a hyperoxidized guanine base produced from oxidation of the mutagenic DNA lesion 7,8-dihydro-8-oxo-2'-deoxguanosine (8-oxoG) by a variety of species including peroxynitrite, singlet oxygen, and the high-valent metals Ir(IV) and Cr(V). In this study, the conformation and thermodynamic stability of a 15-mer DNA duplex containing an Sp lesion are examined using spectroscopic techniques and differential scanning calorimetry (DSC). The Sp lesion does not alter the global B-form conformation of the DNA duplex as determined by circular dichroism spectroscopy. Thermal denaturation experiments find that Sp significantly lowers the thermal stability of the duplex by approximately 20 degrees C. The enthalpies, entropies, and free energies of duplex formation for 15-mers containing guanine, 8-oxoG, and Sp were determined by performing DSC experiments as well as van't Hoff analysis of UV melting spectroscopic data. The thermodynamic stability of the Sp duplex is significantly reduced compared to that of both the 8-oxoG and parent G duplexes, with the thermodynamic destabilization being enthalpic in origin. The thermodynamic impact of the Sp lesion is compared to what is found for other types of DNA base damage and discussed in relation to how the presence of this lesion could affect cellular processes, in particular the recognition and repair of these adducts by the base excision repair enzymes.</div>
</front>
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